Plant regeneration from callus cultures of Piper longum L. by organogenesis

Plant regeneration from callus cultures of Piper longum was achieved through organogenesis. In vitro grown shoots were used as explants for callus induction. Competent callus was initiated around the nodal ring of tissue using Murashige and Skoog medium supplemented with 1.0 mg.l(-1)α- naphthaleneacetic acid and 0.2 mg.l(-1) N(6)-benzyladenine. Optimum growth regulator concentrations for shoot induction and shoot elongation were found to be 0.5 mg.l(-1) indole-3-acetic acid with 1.5 mg.l(-1) benzyladenine, and 0.1 mg.l(-1) indole-3-acetic acid with 0.2 mg.l(-1) benzyladenine, respectively. Elongated shoots were rooted on half-strength Murashige and Skoog medium having 0.1 mg.l(-1) indole3-acetic acid. The rooted plants were successfully established in soil.