Quantifying the local resolution of cryo-EM density maps
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Alp Kucukelbir1, Fred J Sigworth2, Hemant D Tagare3
1Department of Biomedical Engineering, Yale University, New Haven, Connecticut, USA.
Nature Methods
|November 12, 2013
Abstract:
We propose a definition of local resolution for three-dimensional electron cryo-microscopy (cryo-EM) density maps that uses local sinusoidal features. Our algorithm has no free parameters and is applicable to other imaging modalities, including tomography. By evaluating the local resolution of single-particle reconstructions and subtomogram averages for four example data sets, we report variable resolution across a 4- to 40-Å range.

