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Updated: May 6, 2026

Isolation of Atrial Myocytes from Adult Mice
Published on: July 25, 2019
Biological actions of atrial natriuretic factor in flatfish
D Arnold-Reed1, N Hazon, R J Balment
1Department of Physiological Sciences, School of Biological Sciences, University of Manchester, M13 9PT, Manchester, England.
Abstract:
Flounder adapted to seawater were chronically cannulated and received a single i.v. injection of either saline (control) or 10 µg/kg b.w. of human ANF. Compared to controls, ANF significantly reduced (p<0.001) mean arterial blood pressure; full recovery was evident after 4 hours. Blood samples taken at intervals after saline or ANF injection showed that ANF caused a marked increase of 33.7 µg/100 ml in plasma cortisol concentration (p<0.001) 5 hours post injection. The rate of recovery of(22)Na in seawater after a single i.v. injection of 14×10(6) cpm/kg(22)NaCl was significantly increased (p<0.01) following ANF injection compared to controls suggesting that ANF stimulates Na(+) efflux. This observation was confirmed in plaice and dab. The steroidogenic action of ANF and its ability to promote Na(+) efflux are discussed in relation to its potential osmoregulatory role in teleost fish.
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