[Phospholipase D of cereals (author's transl)]

Phospholipase D containing water insoluble fraction was isolated from mature barley; the enzym preparation only had weak phospholipase B activity. The phospholipase D of barley was activated by Ca2+, diethyl ether and sodium dodecyl sulphate (SDS); EDTA inhibited the enzyme to an extent of 10% of the original activity. Diethyl ether and SDS showed and additive effect. Phospholipase D activated by CaC12, diethyl ether and SDS exhibited a sharp optimum at pH 6.6. Lysophosphatidylcholine was hydrolysed much slower than phosphatidylcholine. Diethyl ether and SDS also increased the breakdown of the lysophosphatidylcholine.