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Updated: May 6, 2026
Fabrication of Decellularized Cartilage-derived Matrix Scaffolds
Published on: January 7, 2019
Flexible and elastic scaffolds for cartilage tissue engineering prepared by stereolithography using poly(trimethylene
Sigrid Schüller-Ravoo1, Sandra M Teixeira, Jan Feijen
1MIRA Institute for Biomedical Engineering and Technical Medicine, Department of Biomaterials Science and Technology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands.
Abstract:
The aim of this study is to investigate the applicability of flexible and elastic poly(trimethylene carbonate) (PTMC) structures prepared by stereolithography as scaffolds for cartilage tissue engineering. A three-armed methacrylated PTMC macromer with a molecular weight of 3100 g mol(-1) is used to build designed scaffolds with a pore diameter of 350 ± 12 μm and a porosity of 54.0 ± 2.2%. Upon seeding of bovine chondrocytes in the scaffolds, the cells adhere and spread on the PTMC surface. After culturing for 6 weeks, also cells with a round morphology are present, indicative of the differentiated chondrocyte phenotype. Sulphated glycosaminoglycans and fibrillar collagens are deposited by the cells. During culturing for 6 weeks, the compression moduli of the constructs increases 50% to approximately 100 kPa.
