Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mitochondrial enzyme pathways and their possible role during curing.

C L Walters, I H Burger, G G Jewell

    Zeitschrift Fur Lebensmittel-Untersuchung Und -Forschung
    |July 24, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    PP171. Hospitalized preterm mild preeclamptics: Is there a difference in outcomes between isolated or superimposed disease?

    Pregnancy hypertension·2015
    Same author

    Structure-activity relationships for induction of peroxisomal enzyme activities in primary rat hepatocyte cultures.

    Toxicology in vitro : an international journal published in association with BIBRA·2010
    Same author

    Computer modelling and in vitro tests in the safety evaluation of chemicals-Strategic applications.

    Toxicology in vitro : an international journal published in association with BIBRA·2010
    Same author

    Quantitative structure-activity relationship (QSAR) analysis for a series of rodent peroxisome proliferators: Interaction with the mouse liver peroxisome proliferator-activated receptor alpha (mPPARalpha).

    Toxicology in vitro : an international journal published in association with BIBRA·2010
    Same author

    Molecular modelling of the rat peroxisome proliferator-activated receptor -alpha (rPPARalpha) by homology with the human retinoic acid X receptor alpha (hRXRalpha) and investigation of ligand binding interactions I: QSARs.

    Toxicology in vitro : an international journal published in association with BIBRA·2010
    Same author

    Tumour inhibition with extracts of urine.

    Nature·2010
    Same journal

    [Investigation and evaluation of fumonisin contamination of native and imported cereals].

    Zeitschrift fur Lebensmittel-Untersuchung und -Forschung·1996
    Same journal

    Quantity of K, Ca, Na, Mg, Fe, Cu, Pb, Zn and ashes in DOC Tacoronte-Acentejo (Canary Islands, Spain) musts and wines.

    Zeitschrift fur Lebensmittel-Untersuchung und -Forschung·1996
    Same journal

    Biogenic amines in Spanish beers: differences among breweries.

    Zeitschrift fur Lebensmittel-Untersuchung und -Forschung·1996
    Same journal

    [International Journal of Food Research and Technology].

    Zeitschrift fur Lebensmittel-Untersuchung und -Forschung·1996
    Same journal

    [Degradation of Maillard reaction products by amylolytic enzymes. 3. Inhibition of glucoamylase, alpha-amylase and alpha-glucosidase by heat treated alpha-glucans and melanoidins].

    Zeitschrift fur Lebensmittel-Untersuchung und -Forschung·1996
    Same journal

    [Degradation of Maillard reaction products by amylolytic enzymes. 2. Enzymatic degradation of heat-treated alpha-glucans with and without amino compounds].

    Zeitschrift fur Lebensmittel-Untersuchung und -Forschung·1996
    See all related articles

    Nitrite oxidation of ferrocytochrome c in muscle mitochondria is pH-dependent and can be non-enzymatic at high concentrations. This reaction produces nitrosylferricytochrome c, impacting mitochondrial function.

    Area of Science:

    • Biochemistry
    • Mitochondrial Physiology
    • Nitrite Metabolism

    Background:

    • Ferrocytochrome c plays a crucial role in cellular respiration.
    • Nitrite is known to interact with heme proteins.
    • Skeletal muscle mitochondria are key sites of energy production.

    Purpose of the Study:

    • To investigate the pH-dependent oxidation of ferrocytochrome c by nitrite in skeletal muscle mitochondria.
    • To elucidate the mechanism and products of this reaction under anaerobic conditions.
    • To assess the impact of nitrite on mitochondrial respiration and enzyme systems.

    Main Methods:

    • Spectrophotometric analysis of ferrocytochrome c oxidation.
    • Mitochondrial swelling studies to assess permeability.

    Related Experiment Videos

  • Electron microscopy of mitochondria.
  • Incubation of mitochondria with varying nitrite concentrations under anaerobic and aerobic conditions.

    • Main Results:

    • Ferrocytochrome c oxidation by nitrite is pH-dependent, increasing as pH decreases from 6.7 to 5.6.
    • Nitric oxide-ferricytochrome c complex is a reaction product.
    • Nitrite inhibits aerobic cytochrome oxidase but can cause non-enzymatic ferrocytochrome c oxidation at high concentrations (>20 mM).
    • Mitochondrial permeability to nitrite was confirmed, leading to nitrosylferricytochrome c formation.
    • High nitrite concentrations (>0.3 mM) inhibit mitochondrial enzymes, with non-enzymatic oxidation dominating at 30 mM.

    Conclusions:

    • The oxidation of ferrocytochrome c by nitrite is a direct, non-enzymatic process at high nitrite concentrations.
    • pH significantly influences the rate and mechanism of this reaction in skeletal muscle mitochondria.
    • Nitrite affects mitochondrial integrity and function through both enzymatic and non-enzymatic pathways.