Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mismatch Repair01:20

Mismatch Repair

5.4K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
5.4K
Mismatch Repair01:36

Mismatch Repair

38.2K
Overview
38.2K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.7K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.7K
Bioreactor Controls-III01:22

Bioreactor Controls-III

70
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
70
Homologous Recombination02:31

Homologous Recombination

58.9K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
58.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MOF-Stabilized Pd Nanosheets With Exposed Active Crystal Planes for Efficient Hydrogen Evolution.

ChemSusChem·2026
Same author

Overall Water-Splitting Enabled by Bifunctional NiPd/Pd Heterodimer Fabricated via In Situ Etching-Growth Route.

Inorganic chemistry·2026
Same author

Exploring global research trends in sublingual immunotherapy of allergic rhinitis: a comprehensive bibliometric analysis.

Immunotherapy·2026
Same author

Dual regulatory role of Penicillium oxalicum and Lolium perenne in bauxite residue soilization: Labile organic carbon dynamics and community assembly.

Journal of environmental sciences (China)·2026
Same author

Enhanced <i>Synechococcus</i> Growth Under Extended High-Light and High-Temperature Stress by the F<sub>1</sub>-α-C252Y Mutation in ATP Synthase: ATP Generation and Metabolic Network Remodeling.

Marine drugs·2026
Same author

Mitigation of microbial biocontamination in cyanobacterial ethanol synthesis via alginate encapsulation.

Bioresources and bioprocessing·2026

Related Experiment Video

Updated: May 6, 2026

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
10:07

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

Published on: March 16, 2022

2.4K

Developing controllable hypermutable Clostridium cells through manipulating its methyl-directed mismatch repair

Guodong Luan1, Zhen Cai, Fuyu Gong

  • 1CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

Protein & Cell
|November 12, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed controllable hypermutable Clostridium acetobutylicum cells by inactivating mismatch repair genes and adding an inducible control system. This system allows tunable mutation rates, enhancing microbial evolution studies and industrial applications.

More Related Videos

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
10:32

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Published on: June 10, 2020

3.5K
CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans
09:56

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

Published on: November 14, 2018

12.5K

Related Experiment Videos

Last Updated: May 6, 2026

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
10:07

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

Published on: March 16, 2022

2.4K
Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
10:32

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Published on: June 10, 2020

3.5K
CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans
09:56

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

Published on: November 14, 2018

12.5K

Area of Science:

  • Microbial genetics
  • Bacterial evolution
  • Synthetic biology

Background:

  • Clostridium species are industrially significant bacteria.
  • Understanding and harnessing microbial evolution requires tools like hypermutable cells.
  • No controllable hypermutable systems exist for Clostridium.

Purpose of the Study:

  • To develop a controllable hypermutable cell system for Clostridium acetobutylicum.
  • To enable tunable regulation of mutation rates in bacteria.
  • To enhance the evolvability and adaptability of Clostridium.

Main Methods:

  • Inactivated the mutS/L operon to create hypermutable C. acetobutylicum.
  • Constructed a proofreading control system with an inducible mutS/L operon.
  • Integrated these components into a controllable system (SMBMutC) regulated by anhydrotetracycline (aTc).

Main Results:

  • Achieved over 250-fold increased mutation rates in hypermutable cells.
  • Demonstrated tunable mutation rates in SMBMutC via aTc concentration.
  • Duplication of regulatory modules expanded the mutation rate control range.
  • SMBMutC2 exhibited enhanced survival under butanol stress.

Conclusions:

  • Successfully generated controllable hypermutable Clostridium acetobutylicum cells.
  • The developed system significantly increases microbial evolvability and adaptability.
  • This tool advances microbial evolution research and industrial strain development.