Universal dark quencher based on "clicked" spectrally distinct azo dyes
Arnaud Chevalier1, Julie Hardouin, Pierre-Yves Renard
1Normandie Université , COBRA UMR 6014 & FR 3038, UNIV Rouen, INSA Rouen, CNRS, IRCOF, 1 Rue Tesnières, 76821 Mont-Saint-Aignan Cedex, France , Institut Universitaire de France , 103 Boulevard Saint-Michel, 75005 Paris, France , and Laboratory PBS , UMR 6270 & FR 3038, Bât. Dulong, 76821 Mont-Saint-Aignan Cedex, France.
Abstract:
The first synthesis of an heterotrifunctional molecular scaffold derived from the popular DABCYL azo dye quencher has been achieved. The sequential derviatization of this trivalent azobenzene derivative with two other nonfluorescent azo dyes (Black Hole Quencher BHQ-1 and BHQ-3) and through effective reactions from the "bioconjugation chemistry" repertoire has led to an universal dark quencher (UDQ). This "clicked" poly azo dye is able to turn off an array of fluorophores covering the UV/NIR (300-750 nm) spectral range.
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