Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

9.2K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
9.2K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

915
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
915
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

1.8K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.8K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

10.8K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
10.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Automated Cryo-EM and Supervised Machine Learning Enable Reproducible Characterization of Extracellular Vesicles and Co-Isolating Particles.

Journal of extracellular vesicles·2026
Same author

Nonsense Mutation in <i>USH2A</i> Exon-13 Activates the Innate Immune Response in Müller Glial Cells.

International journal of molecular sciences·2026
Same author

<b>Responses of intestinal organoids to infection by</b> <i><b>Mycobacterium avium</b></i> <b>resemble symptoms observed in Crohn's disease</b>.

Gut microbes·2026
Same author

Benchmarking Lysosome Enrichment Methods: A Guide for Research and Clinical Translation.

Analytical chemistry·2026
Same author

Efficacy prediction of bacteriophage-antibiotic combinations against <i>Staphylococcus aureus</i> biofilms using planktonic bacteria.

Frontiers in microbiology·2026
Same author

KLHL24 mutation drives intermediate filament degradation, mitochondrial dysfunction and fibrosis in heart failure patients.

Cardiovascular research·2025

Related Experiment Video

Updated: May 6, 2026

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management
10:23

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

Published on: June 23, 2023

3.3K

MAVIS: an integrated system for live microscopy and vitrification.

Roman I Koning1, Frank G Faas1, Michael Boonekamp2

  • 1Department of Molecular Cell Biology, Albinusdreef 2, P.O. Box 9600, 2300 RC, Leiden University Medical Center, Leiden, The Netherlands.

Ultramicroscopy
|November 13, 2013
PubMed
Summary

A new system integrates light and electron microscopy for high-resolution imaging of cellular structures. This Microscopy and Vitrification Integrated System (MAVIS) enables precise targeting of dynamic biological events in living cells.

Keywords:
Correlative microscopyCryo-microscopyElectron microscopyLight microscopy

More Related Videos

Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins
08:23

Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins

Published on: September 1, 2013

23.3K
A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva
06:53

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

Published on: May 17, 2020

5.1K

Related Experiment Videos

Last Updated: May 6, 2026

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management
10:23

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

Published on: June 23, 2023

3.3K
Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins
08:23

Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins

Published on: September 1, 2013

23.3K
A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva
06:53

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

Published on: May 17, 2020

5.1K

Area of Science:

  • Cellular and Molecular Imaging
  • Biophysics
  • Microscopy Technology

Background:

  • Cryo-electron microscopy (cryo-EM) offers nanometer-scale 3D reconstructions of biological samples in native states.
  • Limitations in cellular cryo-EM include cryo-fixation artifacts, lack of electron-dense markers, and low inherent contrast.
  • Correlative light and electron microscopy (CLEM) is a strategy to overcome these limitations.

Purpose of the Study:

  • To present a novel integrated system, the Microscopy and Vitrification Integrated System (MAVIS).
  • To enable correlative fluorescence light microscopy and cryo-electron microscopy of living cells and bacteria.
  • To facilitate the targeting of dynamic biological events for subsequent high-resolution imaging.

Main Methods:

  • MAVIS integrates a light microscope with a vitrification device for rapid sample preparation.
  • It allows fluorescence imaging of live cells/bacteria attached to an electron microscopy grid.
  • Vitrification occurs within seconds, preserving cellular structures and dynamics.

Main Results:

  • The MAVIS system successfully combines live-cell fluorescence imaging with rapid vitrification.
  • It enables precise spatial and temporal targeting of cellular events for cryo-EM analysis.
  • Biological examples demonstrate the system's capability for correlative cryo-light and electron microscopy.

Conclusions:

  • MAVIS significantly mitigates limitations of conventional cellular cryo-EM.
  • The system enhances the study of cellular dynamics and molecular localization in native conditions.
  • MAVIS provides a powerful tool for advanced correlative imaging in cell biology.