Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

9.2K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
9.2K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

915
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
915

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association of homocysteine, asymmetric dimethylarginine, and nitric oxide with preeclampsia.

Archives of gynecology and obstetrics·2009
Same author

[Measurement of fine-structure branching ratios for Rb-He optical collisions].

Guang pu xue yu guang pu fen xi = Guang pu·2009
Same author

[The prognostic value of a modified WPSS based on routine laboratory parameters in patients with myelodysplastic syndromes: a preliminary result].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2009
Same author

Lessons learned from the application of a Vietnamese surname list for survey research.

Journal of immigrant and minority health·2009
Same author

Variations of protein levels in human amniotic fluid stem cells CD117/2 over passages 5-25.

Journal of proteome research·2009
Same author

[Effects of Shenwu capsule and its component tetrahydroxystilbene glucoside on expression of neurotrophic factors in lumbar spinal cord of aged rats].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·2009

Related Experiment Video

Updated: May 6, 2026

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
10:01

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Published on: September 8, 2017

7.4K

Immersed transparent microsphere magnifying sub-diffraction-limited objects.

Seoungjun Lee, Lin Li, Zengbo Wang

    Applied Optics
    |November 13, 2013
    PubMed
    Summary
    This summary is machine-generated.

    Microsphere optical nanoscopy (MONS) overcomes the diffraction limit for optical microscopes. This technique uses a barium titanate microsphere to achieve super-resolution imaging in various immersion liquids.

    More Related Videos

    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
    08:53

    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

    Published on: August 15, 2014

    9.1K
    Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
    07:14

    Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

    Published on: April 11, 2025

    1.3K

    Related Experiment Videos

    Last Updated: May 6, 2026

    Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
    10:01

    Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

    Published on: September 8, 2017

    7.4K
    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
    08:53

    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

    Published on: August 15, 2014

    9.1K
    Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
    07:14

    Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

    Published on: April 11, 2025

    1.3K

    Area of Science:

    • Optics and Photonics
    • Materials Science

    Background:

    • Optical microscopy resolution is limited by the diffraction limit (~200 nm for white light).
    • Sub-diffraction-limited imaging is crucial for observing nanoscale structures.

    Purpose of the Study:

    • To demonstrate super-resolution imaging using microsphere optical nanoscopy (MONS).
    • To evaluate MONS performance in different immersion media.

    Main Methods:

    • Utilized a 100 μm barium titanate (BaTiO3) glass microsphere with a standard optical microscope.
    • Performed imaging experiments in water, 40% sugar solution, and microscope immersion oil.
    • Analyzed imaging performance and resolution experimentally.

    Main Results:

    • Achieved sub-diffraction-limited imaging of objects.
    • Demonstrated MONS capability with halogen light in three distinct immersion liquids.
    • Compared image magnification and resolution across different media.

    Conclusions:

    • MONS technique effectively overcomes the diffraction limit.
    • Immersion liquids play a significant role in enhancing super-resolution imaging performance.
    • Mie theory calculations provide insights into the underlying physics of MONS.