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Related Experiment Videos

A new "marker" protein for astrocytes.

R Singh, B Singh, S K Malhotra

    Bioscience Reports
    |January 1, 1986
    PubMed
    Summary

    Researchers developed a new monoclonal antibody (Mab J1-31) that identifies a novel protein in astrocytes, a type of brain cell. This discovery aids in understanding astrocyte function and brain pathology.

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    Area of Science:

    • Neuroscience
    • Immunology
    • Cell Biology

    Background:

    • Astrocytes are crucial glial cells in the central nervous system.
    • Glial fibrillary acidic protein (GFAP) is a well-established marker for astrocytes.
    • Identifying novel astrocyte-specific proteins can advance our understanding of brain function and disease.

    Purpose of the Study:

    • To produce and characterize a new monoclonal antibody (Mab J1-31) targeting a novel protein in astrocytes.
    • To investigate the cellular localization and characteristics of the antigen recognized by Mab J1-31.
    • To differentiate the Mab J1-31 recognized antigen from GFAP.

    Main Methods:

    • Production of monoclonal antibody (Mab J1-31) using mouse immunization with human brain homogenate.
    • Double-label immunofluorescence microscopy on human, rabbit, and rat brain cryostat sections.
    • Immunoprecipitation and SDS-PAGE with autoradiography.
    • Immunoelectron microscopy.

    Main Results:

    • Mab J1-31 stained cells positive for glial fibrillary acidic protein (GFAP) in human, rabbit, and rat brains.
    • Staining by Mab J1-31 was independent of GFAP.
    • Immunoprecipitation identified a single 30K protein band.
    • Immunoelectron microscopy localized the antigen to the cytoskeleton.

    Conclusions:

    • Mab J1-31 recognizes a novel protein distinct from GFAP.
    • This new protein is present in astrocytes.
    • The Mab J1-31 antibody is a valuable tool for studying astrocyte biology and potential roles in neurological conditions.

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