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Complement System

Complement System

The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...

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Related Experiment Video

Updated: May 6, 2026

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

Published on: July 31, 2012

Expression and purification methods for the production of recombinant human complement component C2.

Angela W Norton¹, Paolo G V Martini, Lynette C Cook

¹Department of Biomolecular Research and Technologies, Shire Human Genetic Therapies, Cambridge, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|November 13, 2013

Summary

Researchers developed a method to produce recombinant human complement component C2 (rhC2) protein for scientific research. This rhC2 protein

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Laboratory Scale Production and Purification of a Therapeutic Antibody

Laboratory Scale Production and Purification of a Therapeutic Antibody

Published on: January 24, 2017

Area of Science:

Immunology
Protein Biochemistry

Background:

Human complement component C2 (hC2) is essential for the classical complement pathway.
Recombinant protein production is crucial for detailed functional studies.

Purpose of the Study:

To establish a reliable method for producing recombinant human C2 (rhC2) protein.
To characterize the purity, stability, and enzymatic activity of the produced rhC2.

Main Methods:

Cloning of hC2 cDNA and insertion into a mammalian expression vector.
Transient transfection of mammalian cells for protein expression.
Purification of rhC2 using cation exchange and affinity chromatography.
Characterization of rhC2 purity, stability, and activity via ELISA assays.

Main Results:

Successfully produced and purified rhC2 protein.
Demonstrated the enzymatic activity of rhC2 in complement activation assays.
Confirmed the utility of rhC2 in studying complement pathway function.

Conclusions:

The described method provides a viable source of functional rhC2 for research.
rhC2 is a valuable tool for investigating the classical complement pathway and related immunological processes.