Search research articles

Search research articles

Related Experiment Video

Updated: May 6, 2026

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Published on: October 19, 2018

Purification and characterization of human and mouse complement C3.

Marieta M Ruseva¹, Meike Heurich

¹Department of Medicine, Centre for Complement and Infl ammation research, Imperial College, London, UK.

Methods in Molecular Biology (Clifton, N.J.)

|November 13, 2013

Summary

This study details the purification of complement component C3 from plasma. Purified C3 is then assessed for biochemical and functional characteristics using standard laboratory assays.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Targets for disease modification in schizophrenia: New findings add to evidence for the involvement of the immune complement system.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Complement C4, C4A and C4a - What they do and how they differ.

Brain, behavior, & immunity - health·2024

Same author

Plasma complement and coagulation proteins as prognostic factors of negative symptoms: An analysis of the NAPLS 2 and 3 studies.

Brain, behavior, and immunity·2024

Same author

Proteomic Biomarkers for the Prediction of Transition to Psychosis in Individuals at Clinical High Risk: A Multi-cohort Model Development Study.

Schizophrenia bulletin·2024

Same author

Differential expression of haptoglobin in individuals at clinical high risk of psychosis and its association with global functioning and clinical symptoms.

Brain, behavior, and immunity·2024

Same author

Complement and coagulation crosstalk - Factor H in the spotlight.

Immunobiology·2023

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Area of Science:

Immunology
Biochemistry

Background:

Complement component C3 is the most abundant plasma protein in the complement system.
C3 plays a central role in all three complement activation pathways and amplification.
It is a key target for extensive research in immunology.

Purpose of the Study:

To describe a method for purifying complement component C3 from human and mouse plasma.
To outline the assessment of biochemical and functional characteristics of purified C3.

Main Methods:

Protein precipitation was used for initial C3 isolation from plasma.
Ion exchange chromatography and gel filtration were employed for further purification.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and hemolysis assays were used for characterization.

More Related Videos

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

Depletion of Specific Cell Populations by Complement Depletion

Depletion of Specific Cell Populations by Complement Depletion

Published on: February 5, 2010

Related Experiment Videos

Last Updated: May 6, 2026

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Published on: October 19, 2018

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

Depletion of Specific Cell Populations by Complement Depletion

Depletion of Specific Cell Populations by Complement Depletion

Published on: February 5, 2010

Main Results:

A protocol for purifying C3 from both human and mouse plasma is presented.
Biochemical properties of C3 were analyzed using SDS-PAGE.
Functional complement activity of purified C3 was confirmed via hemolysis assays.

Conclusions:

The described methods allow for the successful purification and characterization of complement component C3.
These techniques are essential for studying the role of C3 in complement-mediated biological processes.
The hemolysis assay remains a standard method for evaluating complement activity.