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Purification and functional analysis of human properdin
Joseph O'Flynn1, Maria C Faber Krol, Mohamed R Daha
1Department of Nephrology, Leiden University Medical Centre, Leiden, The Netherlands.
Methods in Molecular Biology (Clifton, N.J.)
|November 13, 2013
Abstract:
Properdin is a member of the alternative pathway of complement. It is unique in that it is the only known positive regulator of the complement system. Properdin can stabilize and promote complement activation, but in addition is also capable of initiating the alternative pathway, making it a unique protein. This chapter focuses on the methods required for purifying human properdin and testing its functionality.
