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Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung
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Detection of cell membrane-bound CD46 using flow cytometry.

Martin Kolev1, Claudia Kemper

  • 1Division of Transplantation Immunology and Mucosal Biology, MRC Centre for Transplantation, King's College London, Guy's Hospital, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|November 13, 2013
PubMed
Summary
This summary is machine-generated.

CD46 regulates the complement system and immune responses. A new method using specific antibodies and fluorescence-activated cell sorting (FACS) allows sensitive monitoring of CD46 expression on cell surfaces.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD46 is a key regulator of the complement system, preventing self-tissue damage.
  • CD46 activation also mediates intracellular signals crucial for immune regulation.

Purpose of the Study:

  • To develop a sensitive method for monitoring CD46 expression.
  • To analyze CD46 changes on cells and in tissues.

Main Methods:

  • Utilized specific antibodies targeting CD46.
  • Employed fluorescence-activated cell sorting (FACS) analysis.

Main Results:

  • The described method enables the study of cell surface CD46 expression.
  • Demonstrated feasibility of monitoring CD46 levels.

Conclusions:

  • The FACS-based method provides a sensitive approach for CD46 expression analysis.
  • This technique supports research into CD46's roles in complement regulation and immunity.