Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Single inward rectifier channels in horizontal cells.

R Shingai, F N Quandt

    Brain Research
    |March 26, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Kinetic analyses of alcohol-induced potentiation of the response of GABA(A) receptors composed of alpha(1) and beta(1) subunits.

    Journal of biochemistry·2001
    Same author

    Effects of bisphenol A and its derivatives on the response of GABA(A) receptors expressed in Xenopus oocytes.

    Bioscience, biotechnology, and biochemistry·2001
    Same author

    Calcium inhibits willardiine-induced responses in kainate receptor GluR6(Q)/KA-2.

    Neuroreport·2001
    Same author

    Adrenocorticotropic hormone activates an outward current in cultured mouse peritoneal macrophages.

    Journal of neuroimmunology·2001
    Same author

    Upregulation of Kv1.3 K(+) channels in microglia deactivated by TGF-beta.

    American journal of physiology. Cell physiology·2000
    Same author

    Durations and frequencies of free locomotion in wild type and GABAergic mutants of Caenorhabditis elegans.

    Neuroscience research·2000
    Same journal

    IGFBP3 and UBE2C are associated with protein modification pathways and serve as prognostic markers in glioma.

    Brain research·2026
    Same journal

    Targeting neurodevelopmental miR132-3p promotes neuroprotection and axon regeneration after optic nerve injury in mice.

    Brain research·2026
    Same journal

    Variability in acoustic startle response and prepulse inhibition across adulthood in Fragile X messenger ribonucleoprotein 1 knockout mice.

    Brain research·2026
    Same journal

    Transcriptome-guided modeling reveals insulin-related metabolic dysfunction in SCA3 mouse cerebellum.

    Brain research·2026
    Same journal

    Intranasal stromal cell-derived factor-1α mitigates parkinsonian deficits via dual modulation of neuroinflammation and gut microbiota in MPTP-induced models.

    Brain research·2026
    Same journal

    Emotions, the amygdala, and the right hemisphere.

    Brain research·2026
    See all related articles

    Inward rectifier channels in goldfish retinal horizontal cells were identified. Their opening is voltage-sensitive, increasing with hyperpolarization, crucial for cell function.

    Area of Science:

    • Neuroscience
    • Cellular Physiology
    • Retinal Physiology

    Background:

    • Horizontal cells play a critical role in retinal signal processing.
    • Inward rectification is a key electrical property of many cell types, including retinal neurons.
    • The specific ion channels mediating inward rectification in horizontal cells were not fully characterized.

    Purpose of the Study:

    • To identify and characterize the ion channels responsible for inward rectification in goldfish retinal horizontal cells.
    • To elucidate the biophysical properties and voltage-dependence of these inward rectifier channels.

    Main Methods:

    • Utilized the patch clamp technique on isolated goldfish retinal horizontal cells.
    • Analyzed ion selectivity, channel gating kinetics, and responses to external blocking ions.

    Related Experiment Videos

  • Investigated the effects of varying potassium (K+) and sodium (Na+) concentrations and membrane potentials.

    • Main Results:

    • Identified inward rectifier channels with a single channel conductance of 20 pS in 125 mM external K+.
    • Demonstrated high K+ permeability and low Na+ permeability for these channels.
    • Showed that channel opening probability increases with hyperpolarization due to a voltage-sensitive opening rate.

    Conclusions:

    • The inward currents in goldfish horizontal cells are mediated by inward rectifier channels.
    • These channels exhibit distinct ion selectivity and voltage-dependent gating.
    • The voltage sensitivity of the opening rate, not the closing rate, underlies the inward rectification phenomenon in these cells.