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Published on: March 28, 2025
[Bulky DNA adducts in human sperm correlate with semen parameters and sperm apoptosis]
Gui-Xiang Ji1, Sheng-Min Wu, Ji-Ning Liu
1Nanjing Research Institute of Environmental Science, Nanjing, Jiangsu 210042, China. jgx@nies.org
Objective:
To explore the correlation of sperm polycyclic aromatic hydrocarbons (PAH)-DNA adducts with semen quality and sperm apoptosis.
Methods:
We collected semen samples from 433 infertile Chinese men, detected sperm PAH-DNA adducts using immunofluorescence staining and flow cytometry, and determined the rate of sperm apoptosis by TUNEL.
Results:
Multiple linear regression analysis showed that the level of sperm PAH-DNA adducts was correlated negatively with sperm concentration (beta = -0.632), total sperm count (beta = -0.830) and sperm motility (beta = -9.647), but positively with the rate of sperm apoptosis (beta = 0.130).
Conclusion:
Sperm PAH-DNA adducts are evidently correlated with semen quality and sperm apoptosis, and play an important role in the evaluation of male productivity.
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