Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Poiseuille flow method for measuring cell-to-cell adhesion.

M Duszyk, J Doroszewski

    Cell Biophysics
    |April 1, 1986
    PubMed
    Summary

    This study models cell aggregation in flow, finding that cell adhesiveness depends on flow conditions and molecular interactions. We identify key factors influencing cell capture efficiency for better understanding of cell adhesion dynamics.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Polystyrene nanoparticles activate ion transport in human airway epithelial cells.

    International journal of nanomedicine·2011
    Same author

    4-Chloro-benzo[F]isoquinoline (CBIQ) activates CFTR chloride channels and KCNN4 potassium channels in Calu-3 human airway epithelial cells.

    British journal of pharmacology·2004
    Same author

    Coupling of CFTR-mediated anion secretion to nucleoside transporters and adenosine homeostasis in Calu-3 cells.

    The Journal of membrane biology·2003
    Same author

    CFTR and lysozyme secretion in human airway epithelial cells.

    Pflugers Archiv : European journal of physiology·2002
    Same author

    Expression and functional characterization of CFTR in mast cells.

    Journal of leukocyte biology·2002
    Same author

    Regulation of K(+) current in human airway epithelial cells by exogenous and autocrine adenosine.

    American journal of physiology. Cell physiology·2001

    Area of Science:

    • Biophysics
    • Fluid Dynamics
    • Cell Biology

    Background:

    • Cell aggregation is crucial in biological processes.
    • Understanding cell adhesion in flow is vital for various applications.
    • Existing models often simplify the complex interactions involved.

    Purpose of the Study:

    • To develop a theoretical framework for cell aggregation in Poiseuille flow.
    • To quantify cell adhesiveness using capture efficiency.
    • To analyze factors influencing both nonspecific and specific cell-cell interactions.

    Main Methods:

    • Theoretical modeling of cell aggregation dynamics.
    • Application of the DLVO theory for cell adhesion.
    • Analysis of capture efficiency dependence on various physical and chemical parameters.

    Main Results:

    • Capture efficiency is determined for nonspecific and specific cell interactions.
    • Conditions for maximal cell adhesion are identified using DLVO theory.
    • Dependence of capture efficiency on shear rate, cell size, surface potential, Hamaker constant, and medium composition is analyzed.

    Conclusions:

    • The study provides a comprehensive theoretical description of cell aggregation in flow.
    • Key parameters influencing cell adhesion and capture efficiency are elucidated.
    • The findings offer a method to determine receptor diffusion constants from cell aggregation measurements.

    Related Experiment Videos