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Updated: May 6, 2026

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
Published on: July 27, 2013
Amphisomes: out of the autophagosome shadow?
Jana Sanchez-Wandelmer1, Fulvio Reggiori
1Department of Cell Biology and Institute of Biomembranes, University Medical Centre Utrecht, Utrecht, The Netherlands.
Abstract:
Amphisomes are intermediate organelles, formed during autophagy through the fusion between autophagosomes and endosomes. Complex multivesicular vacuoles that resemble amphisomes have been observed in various cell types, but whether they have cellular roles other than being a precursor structure is still enigmatic. While autophagy-related (ATG) proteins interact with the endocytic pathways in other processes different from autophagy, Patel and colleagues now report that these factors come together to generate amphisome-like compartments that regulate mucin secretion in goblet cells.
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