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Updated: May 6, 2026

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
Published on: July 29, 2019
Denaturing gradient gel electrophoresis identifies genomic DNA polymorphism with high frequency in maize
G E Riedel1, S L Swanberg, K D Kuranda
1Genetics Institute, Inc., 87 Cambridge Park Drive, 02140, Cambridge, MA, USA.
Abstract:
We have used denaturing gradient gel electrophoresis (DGGE) to identify genomic DNA polymorphism in maize (Zea mays L.). DGGE probes detect polymorphism in maize at a frequency comparable to the incidence of restriction fragment length polymorphism (RFLP). Probes identifying polymorphism were mapped to maize chromosome arms by utilizing DGGE and maize lines carrying B-A chromosomal translocations. The methods for library construction, probe screening, and genome analysis, described here for maize, can also be applied to the genomic analysis of other organisms.
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