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Related Experiment Video

Updated: May 6, 2026

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
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The DAP-kinase interactome.

Shani Bialik1, Adi Kimchi

  • 1Department of Molecular Genetics, Weizmann Institute of Science, 76100, Rehovot, Israel.

Apoptosis : an International Journal on Programmed Cell Death
|November 14, 2013
PubMed
Summary

Death-associated protein kinase (DAPK) regulates cellular activities through its interactome. This review clarifies DAPK regulation and its diverse cellular effects, including apoptosis and cytoskeletal functions.

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Death-associated protein kinase (DAPK) is a Ca(2+)/calmodulin-regulated kinase.
  • DAPK is involved in multiple cellular processes, including cell death, motility, and signaling cascades.
  • DAPK function is regulated by intramolecular signaling, protein interactions, and ubiquitin-dependent systems.

Purpose of the Study:

  • To review the DAPK interactome, encompassing its substrates, effectors, and regulators.
  • To elucidate the regulatory mechanisms governing DAPK activity.
  • To clarify how DAPK mediates its diverse cellular effects.

Main Methods:

  • Literature review of studies on DAPK substrates, interacting proteins, and regulatory pathways.
  • Analysis of DAPK's role in various cellular processes like apoptosis, autophagy, and necrosis.

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  • Examination of DAPK's catalytic-dependent and independent functions.

    • Main Results:

    • DAPK phosphorylates various kinases (e.g., ZIP-kinase, PKD) and proteins (e.g., Pin1, myosin II, tropomyosin).
    • DAPK mediates cytoskeletal functions, cell death pathways (apoptosis, autophagy, necrosis), and signaling.
    • DAPK also exhibits catalytic-independent functions, activating proteins like pyruvate kinase M2 and NLRP3.

    Conclusions:

    • The DAPK interactome network is crucial for understanding its regulation and cellular functions.
    • DAPK plays a multifaceted role in cellular physiology, impacting diverse pathways.
    • Further research is needed to establish the physiological significance of all identified DAPK substrates and interacting proteins.