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Efficient gene introduction into rice by electroporation and analysis of transgenic plants: use of electroporation
Y Tada1, M Sakamoto, T Fujimura
1Plant Biotechnology Department, Life Science Institute, Mitsui Toatsu Chemicals, Inc, 1144 Togo, 297, Mobara, Japan.
Abstract:
We have developed a method for reproducibly obtaining transgenic rice at a high frequency (10(-4)): electroporation with a buffer in which chloride ions are replaced with organic acids. Co-transformation frequencies of the β-glucuronidase (GUS) and hygromycin phosphotransferase (HPT) genes located on two separate plasmids were higher than 50%. Transgenic rice plants contained multiple copies of introduced genes integrated into their genomes in a complex manner. GUS enzyme activity was not proportional to gene copy number. Introduced HPT genes were detected and expressed in the progeny of transformants.
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