Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioreactor Controls-III01:22

Bioreactor Controls-III

70
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
70

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Environmental impact of using specialty feed ingredients in swine and poultry production: A life cycle assessment.

Journal of animal science·2016
Same author

[Breeding rye varieties from inbred lines : I. Selfing-proportions in polycross progenies].

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2014
Same author

[Restoration of pollen fertility to cytoplasmic male sterile rye].

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2014
Same author

[Applied genetic studies on cytoplasmic pollen sterility in winter rye].

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2014
Same author

Epistasis in maize (Zea mays L.) : 2. Genetic effects in crosses among early flint and dent inbred lines determined by three methods.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Molecular analysis of mitochondrial DNA from rye (Secale cereale L.).

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

Related Experiment Video

Updated: May 6, 2026

A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates
11:48

A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates

Published on: October 28, 2021

3.2K

Methodical improvements in rye anther culture.

T Flehinghaus1, S Deimling, H H Geiger

  • 1University of Hohenheim, Institute of Plant Breeding, Population Genetics, and Seed Science, P.O. Box 700 562/762, W-7000, 70, Stuttgart, Germany.

Plant Cell Reports
|November 14, 2013
PubMed
Summary

Improving rye anther culture is crucial for crop improvement. Researchers enhanced regeneration by substituting sucrose with maltose and applying cold treatment, achieving 20% green plants.

More Related Videos

Efficient Regeneration-based Agrobacterium-Mediated Transformation of an Asexual Amphibious Brassicaceae Species, Rorippa aquatica
06:30

Efficient Regeneration-based Agrobacterium-Mediated Transformation of an Asexual Amphibious Brassicaceae Species, Rorippa aquatica

Published on: January 16, 2026

1.1K
Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
14:53

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

11.0K

Related Experiment Videos

Last Updated: May 6, 2026

A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates
11:48

A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates

Published on: October 28, 2021

3.2K
Efficient Regeneration-based Agrobacterium-Mediated Transformation of an Asexual Amphibious Brassicaceae Species, Rorippa aquatica
06:30

Efficient Regeneration-based Agrobacterium-Mediated Transformation of an Asexual Amphibious Brassicaceae Species, Rorippa aquatica

Published on: January 16, 2026

1.1K
Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
14:53

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

11.0K

Area of Science:

  • Plant Science
  • Agricultural Biotechnology
  • Horticulture

Background:

  • Anther culture in rye (Secale cereale L.) faces significant challenges due to very low regeneration capacity.
  • Developing efficient haploid production methods is vital for accelerating breeding programs in rye.

Purpose of the Study:

  • To overcome the low regeneration capacity in rye anther culture.
  • To investigate the effects of carbohydrate source, post-plating temperature, and gelling agent on rye anther culture efficiency.

Main Methods:

  • Utilized doubled haploid (DH) lines, single crosses between DH lines, and a tetraploid rye population.
  • Investigated the impact of different carbohydrate sources (maltose vs. sucrose), post-plating temperature treatments, and gelling agents.
  • Optimized regeneration protocols for Secale cereale L. anther culture.

Main Results:

  • Substitution of maltose for sucrose significantly improved regeneration rates.
  • A post-plating cold treatment on solidified medium led to substantial progress.
  • Achieved high rates of 49% responding anthers and 20% green plants in one single cross.

Conclusions:

  • The study presents a methodical advancement in rye anther culture techniques.
  • Optimized conditions, particularly maltose use and cold treatment, enhance haploid regeneration in rye.
  • These findings offer a promising pathway for more efficient rye breeding via anther culture.