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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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RNA Splicing01:32

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Pre-mRNA Processing02:01

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pre-mRNA Processing02:01

pre-mRNA Processing

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
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Updated: May 6, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
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Cancer-Associated Perturbations in Alternative Pre-messenger RNA Splicing.

Lulzim Shkreta1, Brendan Bell, Timothée Revil

  • 1Département de microbiologie et d'infectiologie, Faculté de Médecine et des sciences de la santé, Université de Sherbrooke, Québec, Canada.

Cancer Treatment and Research
|November 14, 2013
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Summary

Alternative splicing, a process where one gene produces multiple messenger RNAs (mRNAs), is frequently altered in cancer. These cancer-specific splicing changes can drive tumor growth and impact treatment resistance, offering potential diagnostic and therapeutic targets.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • RNA splicing is crucial for producing functional messenger RNAs (mRNAs) from pre-messenger RNA (pre-mRNA) in most human genes.
  • Alternative splicing allows a single pre-mRNA to generate diverse mRNA molecules, significantly expanding proteomic diversity and organismal complexity.
  • Dysregulation of alternative splicing is implicated in various human diseases, including cancer.

Purpose of the Study:

  • To review the functional impact of alternative splicing variants associated with cancer.
  • To explore the molecular mechanisms driving altered splicing decisions in cancer cells.
  • To discuss the potential of alternative splicing as a target for novel anticancer strategies.

Main Methods:

  • Review of existing literature on RNA splicing, alternative splicing, and cancer biology.
  • Analysis of studies investigating cancer-specific splicing profiles and their molecular determinants.
  • Examination of research on the role of splice isoforms in tumorigenesis and therapeutic resistance.

Main Results:

  • Alternative splicing patterns are frequently altered in cancer cells, contributing to tumorigenesis.
  • Cancer-associated splice isoforms can promote cell proliferation, migration, and resistance to apoptosis and anticancer agents.
  • Alterations in splicing profiles can result from mutations affecting splice sites or changes in the expression of splicing regulatory proteins.

Conclusions:

  • Cancer-specific alternative splicing represents a significant factor in cancer biology.
  • Understanding these splicing alterations can lead to improved cancer diagnosis and prognosis.
  • Targeting aberrant alternative splicing pathways holds promise for developing innovative anticancer therapies.