Cyclometalated iridium(III) complexes with deoxyribose substituents
Ayan Maity1, Jung-Suk Choi, Thomas S Teets
1Department of Chemistry, Case Western Reserve University, 10900 Euclid Avenue, Cleveland Ohio 44106 (USA).
Chemistry (Weinheim an Der Bergstrasse, Germany)
|November 14, 2013
Summary
Researchers developed novel optical probes for studying nucleoside transport. These iridium(III) complex-based nucleoside analogues enable noninvasive tracking of nucleoside transporters in cells.
Area of Science:
- Biochemistry
- Chemical Biology
- Cell Biology
Background:
- Enzymatic nucleoside transport is crucial for cellular metabolism.
- A lack of suitable optical probes hinders the study of nucleoside transporters.
- Nucleoside transporters recognize the deoxyribose sugar, with less specificity for nucleobases.
Purpose of the Study:
- To develop novel, noninvasively trackable optical probes for studying nucleoside transport.
- To synthesize nucleoside analogues functionalized with emissive iridium(III) complexes.
- To investigate the utility of these probes in cellular systems.
Main Methods:
- Synthesis of nucleoside analogues by 'clicking' cyclometalated iridium(III) complexes to the C-1 position of deoxyribose.
- Characterization of the luminescent properties of the synthesized complexes (room temperature and 77 K).
- Crystallographic characterization of a representative complex.
- Demonstration of transport and luminescence in cultured human carcinoma (KB3-1) cells.
Main Results:
- Novel nucleoside analogues with visible luminescence and microsecond triplet lifetimes were synthesized.
- The iridium(III) complexes were successfully attached to the deoxyribose moiety.
- The synthesized probes exhibited luminescence at various temperatures.
- Cellular uptake and transport of the probes were confirmed in human carcinoma cells.
Conclusions:
- Developed emissive iridium(III) complex-based nucleoside analogues serve as effective optical probes.
- These probes facilitate noninvasive tracking of nucleoside transport.
- The study provides a new tool for fundamental research on nucleoside transporters and their function.
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