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Updated: May 6, 2026

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR
Published on: July 8, 2025
In vivo NMR for ¹³C Metabolic Flux Analysis
Albrecht Roscher1, Stéphanie Troufflard, Abdelghani Idrissi Taghki
1Génie Enzymatique et Cellulaire, FRE CNRS 3580, Université de Picardie, Amiens Cedex, France.
Abstract:
The use of in vivo NMR within the framework of Metabolic Flux Analysis in plants is presented. In vivo NMR allows to visualize the active metabolic network, to determine metabolic and isotopic steady state and to measure metabolic fluxes which are not necessarily accessible by isotopic steady state (stationary) Metabolic Flux Analysis. The kinetic data can be used as input for dynamic (nonstationary) Metabolic Flux Analysis. Both 1D and 2D NMR methods are employed.
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