Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transduction01:16

Transduction

3.0K
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
3.0K
DNA Bacteriophages01:26

DNA Bacteriophages

1.6K
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
1.6K
Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

50.0K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
50.0K
Viral Replication: Lytic Cycle01:20

Viral Replication: Lytic Cycle

3.0K
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
3.0K
Bacterial Transformation01:33

Bacterial Transformation

52.2K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
52.2K
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

60.5K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
60.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The diabetic wound microenvironment drives emergence and maintenance of CovRS variants in group B <i>Streptococcus</i>.

Infection and immunity·2026
Same author

Skin androgens regulate Staphylococcus aureus pathogenicity via quorum sensing.

Nature microbiology·2026
Same author

A teichoic acid-like wall modification associated with immune suppression is socially regulated in <i>Streptococcus pyogenes</i>.

mBio·2026
Same author

Collagen binding adhesin restricts Staphylococcus aureus skin infection.

Nature communications·2026
Same author

A teichoic acid-like wall modification associated with immune suppression is socially regulated in <i>Streptococcus pyogenes</i>.

bioRxiv : the preprint server for biology·2026
Same author

An antimicrobial daptide from human skin commensal Staphylococcus hominis protects against skin pathogens.

Nature communications·2025

Related Experiment Video

Updated: May 6, 2026

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus
10:39

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

Published on: March 10, 2017

16.2K

Bacteriophage Transduction in Staphylococcus epidermidis.

Michael E Olson1, Alexander R Horswill

  • 1Department of Microbiology, University of Iowa, Iowa City, IA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 14, 2013
PubMed
Summary

Genetic manipulation of Staphylococcus epidermidis is now faster and easier. Bacteriophage transduction allows for efficient transfer of genetic markers and plasmids, advancing S. epidermidis research.

More Related Videos

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes
07:44

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Published on: February 14, 2025

1.7K
A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

8.3K

Related Experiment Videos

Last Updated: May 6, 2026

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus
10:39

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

Published on: March 10, 2017

16.2K
Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes
07:44

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Published on: February 14, 2025

1.7K
A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

8.3K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Genetic manipulation of Staphylococcus epidermidis presents significant challenges.
  • Traditional methods for strain construction are inefficient and labor-intensive.

Purpose of the Study:

  • To improve the efficiency and speed of genetic manipulation in Staphylococcus epidermidis.
  • To facilitate research on a wider range of S. epidermidis strains, including clinically relevant ones.

Main Methods:

  • Utilized bacteriophage transduction for the transfer of genetic material.
  • Applied this method for moving chromosomal genetic markers and plasmids.

Main Results:

  • Bacteriophage transduction significantly increased the speed and ease of S. epidermidis genetic studies.
  • Enabled research beyond a limited number of genetically tractable strains.

Conclusions:

  • Bacteriophage transduction is a key advancement for Staphylococcus epidermidis molecular genetics.
  • Facilitated broader and more in-depth investigations of this important bacterium.