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Lymphocyte function-associated antigen (LFA-1) is involved in B cell activation.
Journal of Immunology (Baltimore, Md. : 1950)
|June 1, 1986
Summary
A monoclonal antibody targeting LFA-1 (leukocyte function-associated antigen 1) profoundly inhibited B cell responses. However, this inhibition was indirect, mediated by monocytes and T cells, not a direct effect on B cells themselves.
Area of Science:
- Immunology
- Cell Biology
Background:
- Leukocyte function-associated antigen 1 (LFA-1) is crucial for T cell immunity.
- The role of LFA-1 in B cell activation remained undocumented.
Purpose of the Study:
- To investigate the presence of LFA-1 on B cells.
- To determine if anti-LFA-1 antibodies inhibit B cell activation.
Main Methods:
- Examined LFA-1 distribution on normal, neoplastic, and EBV-transformed B cells.
- Tested monoclonal anti-LFA-1 antibody (NB-107) for inhibition of B cell mitogenesis.
- Purified B cells and tested NB-107 effects on enriched B cell populations and EBV-transformed lines.
Main Results:
- NB-107 identified a distinct LFA-1 epitope and was present on most B cells but absent on some B lymphoma lines.
- NB-107 significantly inhibited B cell mitogenesis in peripheral blood mononuclear cells (PBMC).
- NB-107 did not directly inhibit purified B cells, EBV-transformed B cells, or T cell proliferation stimulated by phytohemagglutinin.
Conclusions:
- Anti-LFA-1 antibody inhibits B cell mitogenesis indirectly, likely through effects on monocytes and/or T cells.
- LFA-1 does not appear to directly mediate B cell activation or proliferation.