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Related Experiment Video

Updated: May 6, 2026

Using Practice Testing, Public Speaking, and Source Monitoring to Examine the Influences of Learning Strategies and Stress on Episodic Memory
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Using Practice Testing, Public Speaking, and Source Monitoring to Examine the Influences of Learning Strategies and Stress on Episodic Memory

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Combining stressors that individually impede long-term memory blocks all memory processes.

Sarah Dalesman1, Hiroshi Sunada, Morgan Lee Teskey

  • 1Hotchkiss Brain Institute, Department of Physiology and Pharmacology, University of Calgary, Calgary, Canada.

Plos One
|November 14, 2013
PubMed
Summary

Simultaneous environmental stressors like crowding and low calcium block all memory formation in pond snails. Combining stressors that individually impair long-term memory can additively block short, intermediate, and long-term memory.

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Area of Science:

  • Neuroscience
  • Behavioral Ecology
  • Animal Cognition

Background:

  • Stress impacts memory, but real-world stressors often occur concurrently.
  • Previous research typically assesses single stressors, overlooking combined effects.

Purpose of the Study:

  • To investigate the combined effects of crowding and low calcium on memory and neural activity in Lymnaea stagnalis.
  • To understand how simultaneous stressors affect memory formation and neural correlates.

Main Methods:

  • Operant conditioning of aerial respiration in pond snails (Lymnaea stagnalis).
  • Measurement of aerial breathing behavior and neural activity of the RPeD1 neuron.
  • Assessment of short-term (STM), intermediate-term (ITM), and long-term memory (LTM) under control and stress conditions.

Main Results:

  • Combined stressors (crowding and low calcium) abolished all memory formation (STM, ITM, LTM).
  • Individual stressors (crowding or low calcium) impaired LTM but not STM or ITM formation.
  • Neural activity in the RPeD1 neuron correlated with memory formation and behavioral changes.

Conclusions:

  • Concurrent exposure to multiple stressors can have additive detrimental effects on memory, exceeding the impact of individual stressors.
  • The study highlights the importance of considering combined environmental stressors in understanding memory impairments.