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Related Experiment Video

Updated: May 6, 2026

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

Published on: December 30, 2021

Storage time affects umbilical cord blood viability.

Martin G Guttridge¹, Teck Guan Soh, Heli Belfield

¹NHS Blood and Transplant, Bristol, UK.

|November 15, 2013

Summary

Extending cryopreservation delays for cord blood (CB) negatively impacts cell viability. This study shows that longer storage before freezing reduces the viability of CD45+ and CD34+ cells, crucial for successful transplantation.

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Area of Science:

Hematology
Transplantation Biology
Cell Preservation

Background:

Cryopreserved umbilical cord blood (CB) is a vital cell source for hematopoietic stem cell transplants.
Delays in cryopreservation processing can compromise cell viability and engraftment potential.

Purpose of the Study:

To investigate the impact of extended precryopreservation storage (up to 3 days) on CB cell viability.
To determine if current storage time limits can be safely extended.

Main Methods:

Assessed viability of CD45+ and CD34+ cells in 28 CB donations over 3 days using flow cytometry.
Utilized 7-aminoactinomycin D (7-AAD) and annexin V staining to measure cell viability before and after cryopreservation.

Main Results:

CD34+ cell viability remained high (>92%) before cryopreservation over 3 days.
CD45+ cell viability significantly decreased by Day 3 (p<0.0001).
Post-cryopreservation, CD34+ cell viability decreased by ~5% (7-AAD) to ~7% (annexin V) per extra day of storage.

Conclusions:

Extended precryopreservation storage time adversely affects the viability of CD34+ and CD45+ cells.
Current precryopreservation storage times should be maintained to ensure optimal cell viability for transplantation.