Related Experiment Video

Updated: May 6, 2026

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen
19:44

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen

Published on: May 30, 2012

18.2K

Interferon-alpha and interferon-gamma modulate Fas-mediated apoptosis in mitomycin-C-resistant human Tenon's

Xiao Yang Wang1, Jonathan G Crowston, Andrew J R White

  • 1Centre for Vision Research, Westmead Millennium Institute, The University of Sydney, Sydney, New South Wales, Australia.

Clinical & Experimental Ophthalmology
|November 15, 2013
PubMed
Abstract

Insights

Interferon-alpha and gamma restore sensitivity to Fas-mediated apoptosis in mitomycin-C-resistant cells. This offers a potential alternative anti-fibrotic strategy for trabeculectomy surgery.

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Pharmacology

Background:

  • Trabeculectomy is a surgical procedure to treat glaucoma.
  • Mitomycin-C is commonly used to prevent fibrosis after trabeculectomy, but resistance can develop.
  • Investigating alternative anti-fibrotic agents is crucial for improving surgical outcomes.

Purpose of the Study:

  • To evaluate interferon-alpha and interferon-gamma as potential anti-fibrotic agents.
  • To determine if these interferons can sensitize mitomycin-C-resistant Tenon's fibroblasts to Fas-mediated apoptosis.
  • To explore their use as an alternative to mitomycin-C in trabeculectomy.

Main Methods:

  • Utilized a mitomycin-C-resistant human Tenon's fibroblast cell line.
  • Pretreated cells with interferon-alpha and/or interferon-gamma.
  • Stimulated cells with an agonistic Fas antibody to induce cell death.
  • Analyzed apoptosis-related protein expression via flow cytometry and Western blot.

Main Results:

  • Interferon-alpha and gamma upregulated Fas, Fas-associated protein with death domain, and caspase-8 expression.
  • Combined interferon treatment synergistically increased cell death sensitivity.
  • Fas-mediated apoptosis was confirmed by blocking effects with an anti-Fas antibody and caspase inhibitors.

Conclusions:

  • Interferon-alpha and interferon-gamma can re-sensitize mitomycin-C-resistant cells to Fas-mediated apoptosis.
  • This mechanism involves enhanced death-inducing signaling complex formation.
  • These findings suggest a novel anti-fibrotic strategy for trabeculectomy.
Keywords:
Fasapoptosisglaucomainterferontrabeculectomy

More Related Videos

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer04:20

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer

Published on: February 9, 2024

1.6K
Author Spotlight: Understanding Cytokine-Induced Cell Death in Intestinal Epithelial Cells Using Human Organoids10:03

Author Spotlight: Understanding Cytokine-Induced Cell Death in Intestinal Epithelial Cells Using Human Organoids

Published on: August 2, 2024

1.8K

Related Concept Videos

The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.2K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.2K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.8K
Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
73
Apoptosis01:30

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
12.2K