Inhibitory effects of sweet cherry anthocyanins on the obesity development in C57BL/6 mice
Tao Wu1, Qiong Tang, Zhuoping Yu
1College of Biosystems Engineering and Food Science and.
Abstract:
In the present study, purified sweet cherry anthocyanins (CACN) were evaluated to determine their inhibitory effects on adipocyte differentiation of 3T3-L1 cells and their anti-obesity properties in male C57BL/6 mice fed with high-fat diet (HFD). CACN prevented HFD-induced obesity in C57BL/6 mice. In vivo experiment revealed that 40 and 200 mg/kg of CACN in food reduced the body weight by 5.2% and 11.2%, respectively. CACN supplementation could also reduce the size of adipocytes, leptin secretion, serum glucose, triglyceride, total cholesterol, LDL-cholesterol and liver triglycerides. Furthermore, CACN could effectively reduce the expression levels of IL-6 and TNFα genes, markedly increase the SOD and GPx activity. Our results indicated that CACN slowed down the development of HFD-induced obesity in male C57BL/6 mice.
Insights
Purified sweet cherry anthocyanins (CACN) combat obesity by reducing body weight and adipocyte size in mice. These anthocyanins also improved metabolic markers and reduced inflammation, indicating anti-obesity potential.
Area of Science:
- Nutritional Science
- Biochemistry
- Pharmacology
Background:
- Obesity is a growing global health concern, often exacerbated by high-fat diets (HFD).
- Adipocyte differentiation and associated inflammatory markers play crucial roles in obesity development.
- Natural compounds are being investigated for their potential anti-obesity effects.
Purpose of the Study:
- To evaluate the anti-obesity properties of purified sweet cherry anthocyanins (CACN).
- To investigate the effects of CACN on adipocyte differentiation in vitro and HFD-induced obesity in vivo.
Main Methods:
- In vitro: Assessed CACN's effect on 3T3-L1 cell differentiation.
- In vivo: Administered CACN to male C57BL/6 mice fed an HFD and monitored body weight, adipocyte size, and various serum biomarkers.
- Analyzed gene expression of inflammatory markers (IL-6, TNFα) and antioxidant enzyme activity (SOD, GPx).
Main Results:
- CACN significantly reduced body weight gain in HFD-fed mice (5.2% at 40 mg/kg, 11.2% at 200 mg/kg).
- CACN decreased adipocyte size, leptin secretion, serum glucose, triglycerides, total cholesterol, LDL-cholesterol, and liver triglycerides.
- CACN reduced IL-6 and TNFα gene expression while increasing SOD and GPx activity.
Conclusions:
- Purified sweet cherry anthocyanins demonstrate significant anti-obesity effects in a mouse model of HFD-induced obesity.
- CACN mitigates obesity by reducing adipogenesis, improving lipid profiles, and exerting anti-inflammatory and antioxidant effects.
- These findings suggest CACN as a potential natural therapeutic agent for managing obesity.
