Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel UHMWPE sleeve for stabilizing loose suture anchors in rotator cuff repair.

Archives of orthopaedic and trauma surgery·2026
Same author

Cortical Microinfarcts Are Related to Amyloid Pathology and Cognitive Decline in Spontaneous Intracerebral Hemorrhage.

Journal of stroke·2026
Same author

Time-Dependent Association Between Prehospital Blood Pressure and Outcomes in Acute Spontaneous Intracerebral Hemorrhage.

European journal of neurology·2026
Same author

Taiwan Poststroke Disability Study: A Study Protocol.

Acta neurologica Taiwanica·2026
Same author

Nanobubbles, not microbubbles, enable ultrasound visualization of the perivascular space: A validation and focused ultrasound modulation study.

Ultrasonics·2026
Same author

Defining the reference range of the Plateau-patella angle in Taiwanese patients with osteoarthritis.

Journal of the Formosan Medical Association = Taiwan yi zhi·2026
Same journal

Remodeling TME via feedback-driven photothermal-ferroptosis-immune cascade.

Biomaterials·2026
Same journal

Corrigendum to "Photodynamic therapy produces enhanced efficacy of antitumor immunotherapy by simultaneously inducing intratumoral release of sorafenib" [Biomaterials 2020, 240, 119845].

Biomaterials·2026
Same journal

Mg-integrated octopus-inspired hydrogel dressing enables autonomous adhesion and wound closure for enhanced healing via sequential microenvironment regulation.

Biomaterials·2026
Same journal

Engineering miRNA-223 nanocomplexes via bioorthogonal self-assembly for precision therapy of intervertebral disc degeneration.

Biomaterials·2026
Same journal

Corrigendum to "Enhanced fluorescence imaging guided photodynamic therapy of sinoporphyrin sodium loaded graphene oxide" [Biomaterials 42 (2015) 16442].

Biomaterials·2026
Same journal

An injectable Ce-MnCo LDH nanozyme gel with cascade catalytic activity for acute radiation proctitis in rats.

Biomaterials·2026
See all related articles

Related Experiment Video

Updated: May 6, 2026

Culture of Mouse Neural Stem Cell Precursors
27:44

Culture of Mouse Neural Stem Cell Precursors

Published on: February 25, 2007

19.9K

A neural stem/precursor cell monolayer for neural tissue engineering.

Yi-Chen Li1, Li-Kai Tsai, Jyh-Horng Wang

  • 1Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan.

Biomaterials
|November 15, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed a new medium (medium B) to promote neural stem/precursor cell (NSPC) growth for tissue engineering. This optimized medium supports extensive cell division and controlled differentiation, crucial for neural repair applications.

Keywords:
DifferentiationEpidermal growth factor (EGF)FibronectinMonolayerNeural stem/precursor cells (NSPCs)

More Related Videos

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

11.7K
Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture
06:09

Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture

Published on: May 14, 2015

13.7K

Related Experiment Videos

Last Updated: May 6, 2026

Culture of Mouse Neural Stem Cell Precursors
27:44

Culture of Mouse Neural Stem Cell Precursors

Published on: February 25, 2007

19.9K
Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

11.7K
Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture
06:09

Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture

Published on: May 14, 2015

13.7K

Area of Science:

  • Neuroscience
  • Biomaterials Science
  • Stem Cell Biology

Background:

  • Neural stem/precursor cells (NSPCs) are vital for neural tissue engineering.
  • Controlling NSPC proliferation and differentiation is key for regenerative medicine.
  • Existing methods often lack efficiency in generating NSPC monolayers.

Purpose of the Study:

  • To prepare a confluent monolayer of NSPCs for neural tissue engineering.
  • To investigate the effects of fibronectin and EGF in a defined medium (medium B) on NSPC behavior.
  • To establish a controlled process for NSPC proliferation and differentiation.

Main Methods:

  • NSPCs were isolated from embryonic rat cerebral cortex.
  • Cells were cultured in DMEM/F12 medium supplemented with fibronectin and EGF (medium B).
  • NSPC response, including migration, proliferation, and differentiation, was compared to serum-containing medium.

Main Results:

  • Medium B facilitated neurosphere attachment, extensive cell migration, and enhanced cell division.
  • Medium B culture slowed NSPC differentiation, leading to a confluent monolayer.
  • NSPCs cultured in medium B could form neurospheres or differentiate into neuron-related cells on biomaterials.

Conclusions:

  • A two-stage process using medium B followed by a neuronal differentiation medium was proposed for wound healing and nerve conduit preparation.
  • This approach allows manipulation of NSPC proliferation and MAP2-positive neuron differentiation.
  • The findings provide a method for controlling NSPC behavior on biomaterials for neuroscience research.