JH1 peptide induces antibodies to a common immunoglobulin determinant as detected by cell-binding analysis

In order to more accurately determine the distribution of antigenic determinants detected by antisera to hypervariable-region and JH1 peptides, we measured the frequency of lymphocytes stained with these sera by flow cytometry. None of the sera specific for HV1, HV2 or HV3 peptides stained significant numbers of lymphocytes, but those specific for JH1 reacted with nearly all B-cells.