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Updated: May 6, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Statistical sampling of bacterial strains and its use in bacterial diversity measurement
1Ecologie et Biochimie microbiennnes du milieu marin, CNRS ER 223, Universite de Provence, 3 Place Victor Hugo, F-13331, Marseille Cedex 3, France.
Abstract:
The cultural bacterial strains of two sediment samples, i.e., 260 strains, were submitted to numerical taxonomy to determine "ecological profiles." From these profiles several calculations of bacterial diversity were done with increasing number of strains (between 10 and 130). Studying 20-30 strains was sufficient to obtain a diversity of bacterial community.Number of tests could be reduced from 62 to 30 without any influence on bacterial diversity. Similarity between studied tests was shown by using numerical taxonomy.
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