Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

6.9K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
6.9K
Primary Lymphoid Organs01:16

Primary Lymphoid Organs

9.6K
Primary lymphoid organs are pivotal in the formation, development, and maturation of lymphocytes, the white blood cells that serve as the backbone of our immune system. This crucial function underscores their fundamental role in maintaining our overall health and immunity. The two primary lymphoid organs of prime importance are the red bone marrow and the thymus.
The red bone marrow is a soft, spongy tissue nestled in the interior of long bones such as the humerus and femur. It is the site...
9.6K
Secondary Lymphoid Organs01:15

Secondary Lymphoid Organs

7.3K
Secondary organs, including lymph nodes, the spleen, and mucosa-associated lymphoid tissue (MALT), work harmoniously to protect us from disease and infection.
The spleen is a vital organ in the lymphatic system, nestled in the upper left side of the abdomen. It is composed of two primary regions: the red pulp and the white pulp, each having distinct functions. The red pulp performs a significant role in blood filtration. It efficiently purges the blood of old or damaged red blood cells and...
7.3K
Lymphoid Cells and Tissues01:18

Lymphoid Cells and Tissues

3.4K
Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
3.4K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

13.7K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
13.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Smartphone App-based Malignant Hematology Curriculum for Internal Medicine Residents: A Pilot Study.

Journal of cancer education : the official journal of the American Association for Cancer Education·2026
Same author

Mutation-specific NPM1 immunohistochemistry is a sensitive method for detecting residual disease in mid- and postinduction bone marrows: comparison of NPM1 immunohistochemistry, NPM1 real-time polymerase chain reaction, and flow cytometric studies.

American journal of clinical pathology·2026
Same author

T-prolymphocytic leukemia with three distinct immunophenotypic subsets.

Virchows Archiv : an international journal of pathology·2026
Same author

Heptazine Based Photo-Programmable Azobenzene-Amino Acid Nanotubes for Augmented Drug Delivery and Chemo-Photodynamic Therapy in Glioma.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

The Hidden Dangers of Vaping and the Need for Community Action: An Indian Perspective.

International journal of preventive medicine·2026
Same author

Community Health Workers Intervention in Artificial Intelligence-Integrated Personalized Medicine: A Holistic Approach to Polycystic Ovary Syndrome Management.

International journal of preventive medicine·2026

Related Experiment Video

Updated: May 6, 2026

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
08:56

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Published on: June 9, 2015

7.9K

Splenic lymphoid subsets with less well-recognized phenotypes mimic aberrant antigen expression.

Nidhi Aggarwal1, Jason Fischer, Steven H Swerdlow

  • 1Division of Hematopathology, Department of Pathology, University of Pittsburgh School of Medicine, 200 Lothrop St, Suite G300, Pittsburgh, PA 15213-2582; craigfe@upmc.edu.

American Journal of Clinical Pathology
|November 15, 2013
PubMed
Summary

Understanding normal lymphoid cell phenotypes is crucial for accurate lymphoma diagnosis using flow cytometry. This study identifies common and less recognized normal subsets in spleen and blood to prevent misinterpretation.

Keywords:
AberrantFlow cytometryPhenotypeSpleen

More Related Videos

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
09:25

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

11.6K
Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches
08:25

Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches

Published on: November 21, 2018

13.2K

Related Experiment Videos

Last Updated: May 6, 2026

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
08:56

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Published on: June 9, 2015

7.9K
Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
09:25

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

11.6K
Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches
08:25

Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches

Published on: November 21, 2018

13.2K

Area of Science:

  • Immunology
  • Hematology
  • Cell Biology

Background:

  • Flow cytometry is vital for lymphoma diagnosis by detecting aberrant antigen expression.
  • Accurate interpretation requires a thorough understanding of normal lymphoid cell phenotypes.

Purpose of the Study:

  • To characterize normal lymphoid subsets in the spleen using 8-color flow cytometry.
  • To identify less recognized normal lymphoid populations that may mimic neoplastic cells.

Main Methods:

  • Analysis of 20 spleens from traumatic rupture using 8-color flow cytometry.
  • Characterization of lymphoid subsets, including CD5+ B cells, CD7- T cells, and CD3 brightγδT cells.
  • Comparison with 20 peripheral blood specimens to assess population proportions.

Main Results:

  • Identified well-recognized subsets like CD5+ B cells and CD7- T cells.
  • Discovered less recognized subsets resembling lymphoma populations: CD5- T cells, CD2- natural killer (NK) cells, and CD7dim+ NK cells.
  • These less recognized subsets were present in all specimens and in peripheral blood, though in smaller proportions.

Conclusions:

  • Familiarity with normal lymphoid subset phenotypes aids in accurate flow cytometry interpretation.
  • Normal expanded subsets can be mistaken for aberrant antigen expression in neoplastic cells, highlighting the importance of this knowledge.