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Published on: November 15, 2013
Measurement of Electron Transport System (ETS) activity in soil
J T Trevors1, C I Mayfield, W E Inniss
1Department of Biology, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada.
Abstract:
Electron transport system (ETS) activity was measured in amended and nonamended soil by measuring the reduction of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT) to iodonitrotetrazolium formazan (INT-formazan), which can be easily extracted with methanol without interference from other compounds found in soil. A high correlation between ETS activity and oxygen consumption was observed. This technique allows rapid quantitative measurements of microbial ETS activity in soil.
