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Updated: May 6, 2026
Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
Published on: February 14, 2020
Quantitative three-dimensional study on the position of the female gametophyte and its constituent cells as a
V T Wagner1, C Dumas, H L Mogensen
1Reconnaissance Cellulaire et Amelioration des Plantes, Université Claude Bernard-Lyon I/La INRA 879 43 Boul. du 11 Novembre 1918, F-69622, Villeurbanne Cedex, France.
Abstract:
The position of the embryo sac in the spikelet and of the embryo sac's constituent cells within the sporophytic tissues of Zea mays was localized by scanning electron microscopy, serial thick sectioning, and computer three-dimensional reconstruction. Within certain limits, the embryo sac is consistently oriented in the same position inside of the spikelet. This information is a prerequisite for successful microinjections into the in situ female cells of Zea mays.
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