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Related Experiment Video

Updated: May 6, 2026

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability
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An aerodynamic drag model for protein ions.

D J Douglas1

  • 1Sciex, 55 Glen Cameron Road, L3T lP2, Thornhill, Ontario, Canada.

Journal of the American Society for Mass Spectrometry
|November 15, 2013
PubMed
Summary

Protein ion energy loss in collision cells was modeled using aerodynamic drag. This approach yielded protein cross sections approximately 0.8 times smaller than previous methods, improving accuracy for ion dynamics.

Area of Science:

  • Physical Chemistry
  • Chemical Physics
  • Ion Physics

Background:

  • Understanding ion energy loss in collision cells is crucial for mass spectrometry.
  • Previous models for protein ion energy loss may lack precision at high Knudsen numbers.

Purpose of the Study:

  • To model protein ion energy loss in inert gas collision cells using aerodynamic drag.
  • To re-evaluate protein cross sections using a refined physical model.

Main Methods:

  • Applied aerodynamic drag model for high Knudsen number regimes.
  • Utilized drag coefficients from gas dynamics literature.
  • Analyzed energy loss data from Covey and Douglas (1993).

Main Results:

  • The aerodynamic drag model provides a new framework for ion energy loss.

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  • Derived protein cross sections were approximately 0.8 times those from the simple collision model.
  • This suggests a refinement in understanding ion-gas interactions.

    • Conclusions:

    • Aerodynamic drag offers a more accurate representation of protein ion energy loss.
    • The refined cross sections enhance the physical interpretation of ion-molecule collisions.
    • This model improves the analysis of ion dynamics in mass spectrometry.