Long-term storage of Pennisetum glaucum (L.) R. Br. pollen

Area of Science:

• Agricultural Science
• Botany
• Plant Physiology

Background:

• Long-term storage of plant genetic material is crucial for crop conservation.
• Pearl millet (Pennisetum glaucum) is a vital cereal crop, particularly in arid regions.
• Effective pollen storage methods are needed to preserve genetic diversity.

Purpose of the Study:

• To determine the long-term viability of pearl millet pollen under different storage conditions.
• To investigate the impact of temperature, moisture content, and container type on pollen viability.
• To identify optimal storage parameters for extended pearl millet pollen preservation.

Main Methods:

• Pearl millet pollen was stored at -18°C and -73°C for up to 2,911 days.
• Pollen moisture content was controlled and varied (below 7.2%).
• Storage containers included glass vials and zip-lock plastic bags.

Main Results:

• Pollen remained viable after 2,615 days at -18°C and 2,911 days at -73°C.
• Viability at -73°C was minimally affected by moisture content (<7.2%) or container type.
• Pollen moisture content was more critical for viability at -18°C than at -73°C.
• Glass vials were superior for long-term storage (>3 years) at -18°C.

Conclusions:

• Pearl millet pollen can be stored long-term at sub-zero temperatures while maintaining viability.
• Storage temperature significantly influences the impact of moisture content and container type on pollen viability.
• Specific storage conditions, including glass vials and controlled moisture, are recommended for optimal long-term preservation of pearl millet pollen.