Related Experiment Video
Updated: May 6, 2026

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts
Published on: July 24, 2012
Evidence for an excited-state reaction contributing to NADH fluorescence
1McCollum-Pratt Institute, Department of Biology, Johns Hopkins University, 21218, Baltimore, Maryland.
Abstract:
The fluorescence of reduced β-nicotinamide adenine dinucleotide (NADH) was monitored as a function of the excitation and emission wavelengths. In aqueous and organic solvents the intensity decay was found to be more heterogeneous than reported earlier. When the ternary complex of NADH with the enzyme (horse liver alcohol dehydrogenase) and substrate analog (iso-butyramide) is formed, three exponents are required to fit the data. The decay-associated spectrum for the shortest lifetime undergoes a sign change from positive at the blue edge of emission to negative at the red edge. This phenomenon is interpreted as an outcome of reversible excited-state reaction leading to the appearance of at least one fluorescent product.
Related Concept Videos
Deactivation Processes: Jablonski Diagram
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Role of Reduced Coenzymes NADH and FADH₂
The Photochemical Reaction Center
Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals
Photoluminescence: Fluorescence and Phosphorescence
A pair of electrons in a...

