Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

16.9K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
16.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Circadian disruption induces sex-specific Alzheimer's pathophysiology and immune cell reprogramming.

bioRxiv : the preprint server for biology·2026
Same author

Effect of transcript length, temperature, and lyophilization on mRNA-lipid nanoparticle stability.

Journal of pharmaceutical sciences·2026
Same author

Integrated transcriptomic and functional analyses reveal that NOX2 inhibition rewires the inflammatory landscape of macrophages.

Frontiers in immunology·2026
Same author

Probing Glycosaminoglycan-Protein Interactions: Applications of Surface Plasmon Resonance.

Biosensors·2026
Same author

Enhanced lentiviral vector recovery and separation using arginine hydrochloride with CIM QA monoliths.

Biotechnology progress·2026
Same author

Brain vascular stability relies on PAK2-cilia-PDGF-BB-HSPGs on basolateral side of endothelium.

Life science alliance·2026

Related Experiment Video

Updated: May 6, 2026

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces
12:04

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

Published on: March 1, 2017

11.1K

Microarray platform affords improved product analysis in mammalian cell growth studies.

Payel Datta1, Luciana Meli2, Lingyun Li3

  • 1Department of Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY.

Biotechnology Journal
|November 15, 2013
PubMed
Summary

A new high throughput (HT) microarray platform effectively assesses cell culture conditions for Chinese hamster ovary (CHO) cells. This method aids in evaluating media additives

Keywords:
3D cell cultureCHO cellsCell-based microarrayHigh throughputMedia optimization

More Related Videos

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

10.0K
Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer
08:20

Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer

Published on: May 21, 2019

5.1K

Related Experiment Videos

Last Updated: May 6, 2026

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces
12:04

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

Published on: March 1, 2017

11.1K
A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

10.0K
Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer
08:20

Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer

Published on: May 21, 2019

5.1K

Area of Science:

  • Biotechnology
  • Cell Culture Technology
  • Bioprocessing

Background:

  • High throughput (HT) platforms offer cost-efficient and rapid screening for cell culture conditions and chemical evaluations.
  • Chinese hamster ovary (CHO) cells are widely used for therapeutic monoclonal antibody production.
  • Optimizing culture conditions is crucial for enhancing cell growth, productivity, and product quality.

Purpose of the Study:

  • To develop and validate a HT cell-based microarray platform for assessing culture conditions in CHO cells.
  • To evaluate the impact of a non-proprietary medium on CHO cell growth, transgene expression, metabolism, and monoclonal antibody production.
  • To investigate the effect of media additives, specifically glutamate, on cellular processes within the microarray and shake flask platforms.

Main Methods:

  • Development of a HT cell-based microarray system with 60-nL cell spots encapsulated in alginate within an 8-well chamber.
  • Culturing a GS/methionine sulphoximine (MSX) CHO cell line producing a therapeutic monoclonal antibody on the microarray and conventional shake flask platforms.
  • Utilizing a non-proprietary medium and analyzing cell growth, transgene expression, metabolism, and antibody glycosylation.

Main Results:

  • The developed non-proprietary medium supported cell growth, monoclonal antibody production, normal glycosylation, and metabolism in both microarray and shake flask platforms.
  • The HT microarray platform demonstrated comparable results to the conventional shake flask system.
  • Addition of 10.3 mM glutamate to the defined base medium induced a lactate metabolism shift in GS/MSX CHO cells in the shake flask.

Conclusions:

  • The developed HT microarray platform is a viable tool for evaluating the impact of media additives on cellular processes like growth, metabolism, and productivity.
  • This platform offers a cost-effective and rapid alternative for optimizing cell culture conditions in biopharmaceutical development.
  • The study validates the use of non-proprietary media and highlights the potential for detailed cellular process analysis using microarray technology.