Related Experiment Video
Updated: May 6, 2026
Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
Published on: October 20, 2014
EBP1, a novel host factor involved in primer binding site-dependent restriction of moloney murine leukemia virus in
Gary Z Wang1, Daniel Wolf, Stephen P Goff
1Integrated Program in Cellular, Molecular and Biophysical Studies, Columbia University, New York, New York, USA.
Abstract:
Mouse embryonic cells are unable to support the replication of Moloney murine leukemia virus (MLV). The integrated viral DNA is transcriptionally silenced, largely due to binding of host transcriptional repressors to the primer binding site (PBS) of the provirus. We have previously shown that a PBS DNA-binding repressor complex contains ZFP809 and TRIM28. Here, we identified ErbB3-binding protein 1 (EBP1) to be a novel component of the ZFP809-TRIM28 silencing complex and show that EBP1 depletion reduces PBS-mediated retroviral silencing.
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