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[Study on sequence characterized amplified region (SCAR) markers of Polygonum capitatum]
Tao Zhou1, Yu Xie, Li-Yan Zhang
1Guiyang college of Traditional Chinese Medicine, Guiyang 550002, China. taozhou88@163.com
Objective:
To establish sequence characterized amplified region markers of Polygonum capitatum.
Method:
The random primer was screened through RAPD to obtain the specific RAPD marker band, and the band was separated, extracted, cloned and sequenced. The specific primers were designed for conventional PCR reaction on the basis of the specific band, and the SCAR marker was acquired.
Result:
Screening from 50 RAPD primer, only C29 primer had 2 specific bands could distinguish P. capitatum from P. nepalense, then 4 pairs of specific primers were designed based on the 2 sequences of RAPD marker bands, and only 1 pair primer (Z1-2) was successfully converted into SCAR marker after repeated tests.
Conclusion:
The Z1-2 primer, could be used as an effective SCAR mark to identify Z300 DNA for P. capitatum. The SCAR mark was established and can be used as a molecular marker to distinguish P. capitatum from P. nepalense
