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Expression of chicken CTCF gene in COS-1 cells and partial purification of CTCF protein
E S Kotova1, I V Sorokina, S B Akopov
1Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia. akser@ibch.ru.
Biochemistry. Biokhimiia
|November 16, 2013
Abstract:
The chicken gene for transcription factor CTCF was expressed in COS-1 mammalian cells. The CTCF protein containing polyhistidine tag was partially purified using metallo-affinity and ion-exchange chromatography. The expressed protein localized in the cell nucleus and was shown to be functionally active in the electrophoretic mobility shift assay and specifically interacted with anti-CTCF antibodies.
