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Serum metabolites of proanthocyanidin-administered rats decrease lipid synthesis in HepG2 cells.

Ligia Guerrero1, Maria Margalef, Zara Pons

  • 1Department of Biochemistry and Biotechnology, Rovira i Virgili University, Tarragona 43007, Spain; Department of Research, Nutrition and Innovation, ALPINA S.A, Bogotá, Colombia.

The Journal of Nutritional Biochemistry
|November 16, 2013
PubMed
Summary
This summary is machine-generated.

Flavonoid-rich extracts from cocoa, pine bark, and grape seeds reduce lipid synthesis in liver cells. Metabolites from grape seed extract in rat serum showed even greater effects on reducing cholesterol and triglycerides.

Keywords:
Cell culturesGrape seed proanthocyanidin extractHepG2 cellsLipid synthesisSerum metabolites

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Area of Science:

  • Nutritional biochemistry
  • Cardiovascular health
  • Cell biology

Background:

  • Regular flavonoid consumption is linked to lower mortality and cardiovascular disease risk.
  • Plasma proanthocyanidins differ significantly from dietary flavonoid sources.
  • In vitro analysis of flavonoid bioactivity requires physiologically relevant conjugates.

Purpose of the Study:

  • To evaluate the impact of cocoa (CCX), Pycnogenol (PYC), and grape seed (GSPE) extracts on lipid homeostasis.
  • To assess the in vitro effect of GSPE proanthocyanidins on lipid synthesis using physiological serum metabolites.

Main Methods:

  • Hepatic human HepG2 cells were treated with CCX, PYC, or GSPE (25 mg/L).
  • Wistar rats received GSPE (1 g/kg), and serum was collected after 2 hours.
  • Serum metabolites were characterized using LC-QqQ/MS(2); effects on cholesterol ester (CE), free cholesterol (FC), and triglycerides (TG) were analyzed.

Main Results:

  • All three extracts significantly decreased de novo lipid synthesis in HepG2 cells.
  • GSPE rat serum metabolites reduced total CE, FC, and particularly TG percentages.
  • The reduction in lipids by GSPE serum metabolites was significantly greater than direct GSPE treatment.

Conclusions:

  • Proanthocyanidin-rich extracts effectively reduce lipid synthesis in liver cells.
  • Physiological metabolites of GSPE in serum exhibit enhanced bioactivity compared to the extract alone.
  • This study demonstrates the in vivo-derived metabolite bioactivity of proanthocyanidins in HepG2 cells.