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Updated: May 6, 2026

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
PCR-restriction fragment length polymorphism analysis using groEL gene to differentiate pathogenic Vibrio species
Muhammad Tofazzal Hossain1, Yu-Ri Kim1, In-Soo Kong1
1Department of Biotechnology, Pukyong National University, Busan, 608-737 Korea.
Abstract:
Important pathogenic Vibrio species were differentiated by PCR-restriction fragment length polymorphism analysis. A 1117-bp groEL gene product was amplified using universal primers and digested using the restriction enzymes NruI or XbaI, revealing unique digestion patterns for each of the 10 Vibrio species, of which 7 were pathogenic in humans, along with 2 other species pathogenic in fish.
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