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Related Concept Videos

Development of the Heart01:27

Development of the Heart

3.9K
The development of the human heart, a crucial organ, commences from the mesoderm on the 18th or 19th day after fertilization. This process initiates in the cardiogenic area, a group of mesodermal cells at the embryo's head end, which evolves into elongated strands known as cardiogenic cords. These cords undergo a transformation to form hollow-centered endocardial tubes.
As the embryo undergoes lateral folding, these paired tubes approach each other, merging into a single primitive heart...
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Development of Blood Vessels01:07

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The development of the vascular system in a fetus is a complex and intricate process that begins as early as 15 to 16 days post-conception. This process starts outside the embryo, specifically in the mesoderm of the yolk sac, chorion, and connecting stalk. Approximately two days later, the formation of blood vessels occurs within the embryo itself.
The initial formation of this system is facilitated by the small amount of yolk present in the ovum and yolk sac. Blood vessels originate from...
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Related Experiment Video

Updated: May 6, 2026

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
06:27

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

Published on: April 17, 2019

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Quantifying function in the early embryonic heart.

Brennan M Johnson, Deborah M Garrity, Lakshmi Prasad Dasi

    Journal of Biomechanical Engineering
    |November 16, 2013
    PubMed
    Summary
    This summary is machine-generated.

    We developed a new method to quantify cardiac function in embryonic zebrafish. This technique allows for precise measurements of blood flow and heart chamber size during early development.

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    Related Experiment Videos

    Last Updated: May 6, 2026

    Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
    06:27

    Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

    Published on: April 17, 2019

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    Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution
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    Functional Cardiac Imaging in Zebrafish Embryos Using Standard Microscopy and Video Analysis: Applications in Environmental and Biomedical Research
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    Functional Cardiac Imaging in Zebrafish Embryos Using Standard Microscopy and Video Analysis: Applications in Environmental and Biomedical Research

    Published on: October 10, 2025

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    Area of Science:

    • Developmental Biology
    • Cardiovascular Research
    • Zebrafish Models

    Background:

    • Congenital heart defects stem from early developmental issues.
    • Abnormal blood flow and cardiac function are implicated in cardiac morphogenesis.
    • Embryonic zebrafish provide excellent optical access for studying heart development.

    Purpose of the Study:

    • To develop and validate a methodology for quantifying cardiac function in embryonic zebrafish.
    • To establish a repeatable method for analyzing heart dynamics in vivo.
    • To enable the study of cardiac function during early embryonic development.

    Main Methods:

    • Utilized bright field microscopy at 1500 frames/s for high-resolution imaging.
    • Quantified blood velocity and luminal diameter at the atrial inlet and AVJ.
    • Employed spatiotemporal plot analysis and control volume analysis for flow rate, stroke volume, and cardiac output estimation.
    • Manipulated heart function in wild-type zebrafish at approximately 55 hours post fertilization.

    Main Results:

    • Demonstrated high repeatability of diameter and flow waveforms at the inlet and AVJ between heartbeats.
    • Successfully quantified key cardiac function parameters including flow rate, retrograde fractions, stroke volume, and cardiac output.
    • Established a robust methodology applicable to early zebrafish development stages.

    Conclusions:

    • A novel, repeatable methodology for quantifying embryonic zebrafish cardiac function has been established.
    • This technique provides valuable insights into normal and potentially abnormal heart development.
    • The developed method is suitable for application in early-stage zebrafish embryos.