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Urease in jack-bean (Canavalia ensiformis (L.) DC) seeds is a cytosolic protein
L Faye1, J S Greenwood, M J Chrispeels
1Department of Biology, C-016, University of California San Diego, 92093, La Jolla, CA, USA.
Abstract:
Urease (EC 3.5.1.5) is abundantly present in the seeds of many species of Leguminosae. There is at present conflicting information in the literature about its subcellular location and status as a glycoprotein. We have made a study of the subcellular location of urease in jack-bean cotyledons using an immunocytochemical approach; in addition, we studied the biosynthesis and glycoprotein nature of the enzyme using several biochemical approaches. All the results are in agreement with the interpretation that the seed urease is not a glycoprotein, is synthesized on free polysomes, and is present in the cytosol of the storage parenchyma cells.
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