Related Experiment Video
Updated: May 6, 2026

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants
Published on: December 16, 2015
Genome specific, highly repeated sequences of Hordeum vulgare: cloning, sequencing and squash dot test
1Department of Genetics, Martin-Luther-Universität, Domplatz 1, DDR-4020, Halle/S., German Democratic Republic.
Abstract:
Highly repeated sequences of nuclear DNA from barley Hordeum vulgare (L.) variety 'Erfa' were cloned. Several clones containing barley specific repeated DNA were analysed by sequence analysis and Southern blot hybridization. The investigated repeats differ from each other in their length, sequence and redundancy. Their length ranges from 36 bp to about 180 bp. The repeats are AT-rich and differ widely in their redundancy within the barley genome. Southern analysis showed that the repeats belong to different repetition complexes. The possibility for utilizing these clones as probes for simple and fast genome analysis is demonstrated in squash dot experiments.
More Related Videos
09:32An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
Published on: November 8, 2017
12:33Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Published on: July 28, 2017
Related Concept Videos
Modern Molecular Taxonomy
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Evolutionary Relationships through Genome Comparisons